Cofactor engineering drives natural product synthesis


Cofactor engineering drives natural product synthesis
Graphical summary. Credit history: Mother nature Chemical Biology (2022). DOI: 10.1038/s41589-022-01014-6

In the past decade, advances in synthetic biology have paved the way toward the sustainable synthesis of advanced all-natural products.

The baking yeast Saccharomyces cerevisiae has been extensively utilised in food marketplace and has turn out to be 1 of the major platforms for making cell factories because of to its robustness, easy cellular engineering and responsible safety.

Lately, a investigation group led by Prof. Zhou Yongjin from the Dalian Institute of Chemical Physics (DICP) of the Chinese Academy of Sciences (CAS), in collaboration with Prof. Zhang Lei from Naval Healthcare College, has developed efficient cofactor engineering methods to derive phenolic acid biosynthesis in yeast.

This review was revealed in Nature Chemical Biology on April 28.

The cofactor metabolic process is tightly regulated and complicatedly distributed in sub-organelles in eukaryotic cell this kind of as yeast. Consequently, it necessitates novel and feasible engineering approaches to cope with the elaborate cofactor regulation.

The scientists developed personalized engineering strategies to boost the source, re-localization, and recycling of cofactors NADPH, Trend(H2), and SAM, which enabled high-amount production of caffeic acid (5.5 g/L) and ferulic acid (3.8 g/L).

“This function reveals the regulation of various cofactors in yeast, especially the distribution of cofactors in between unique organelles in cells, and provides theoretical advice for cofactor engineering,” said Prof. Zhou. “It also presents enough precursors for the economical synthesis of elaborate active all-natural solutions.”

Productive genetic engineering system proven in methylotrophic yeast

More information and facts:
Ruibing Chen et al, Engineering cofactor provide and recycling to drive phenolic acid biosynthesis in yeast, Nature Chemical Biology (2022). DOI: 10.1038/s41589-022-01014-6

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